GrippeCanadaV3, Main, Exploration, bibRecord, 000139

Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.

Identifieur interne : 000139 ( Main/Exploration ); précédent : 000138; suivant : 000140

Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.

Auteurs : Hua Yang [États-Unis] ; Paul J. Carney [États-Unis] ; Vasiliy P. Mishin [États-Unis] ; Zhu Guo [États-Unis] ; Jessie C. Chang [États-Unis] ; David E. Wentworth [États-Unis] ; Larisa V. Gubareva [États-Unis] ; James Stevens

Source :

Journal of virology [ 1098-5514 ] ; 2016.

RBID : pubmed:27053557

Descripteurs français

English descriptors

KwdEn :
- Animals, Animals, Wild (virology), Asia (epidemiology), Canada (epidemiology), Disease Outbreaks, Europe (epidemiology), Hemagglutinin Glycoproteins, Influenza Virus (chemistry), Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (metabolism), Humans, Influenza A Virus, H5N1 Subtype (chemistry), Influenza A Virus, H5N1 Subtype (enzymology), Influenza A Virus, H5N1 Subtype (genetics), Influenza A Virus, H5N2 Subtype (chemistry), Influenza A Virus, H5N2 Subtype (enzymology), Influenza A Virus, H5N2 Subtype (genetics), Influenza A Virus, H5N8 Subtype (chemistry), Influenza A Virus, H5N8 Subtype (enzymology), Influenza A Virus, H5N8 Subtype (genetics), Influenza in Birds (epidemiology), Influenza in Birds (virology), Influenza, Human (virology), Neuraminidase (chemistry), Neuraminidase (genetics), Neuraminidase (metabolism), North America (epidemiology), Phylogeny, Poultry, Reassortant Viruses.
MESH :
- chemical , chemistry : Hemagglutinin Glycoproteins, Influenza Virus, Neuraminidase.
- chemistry : Influenza A Virus, H5N1 Subtype, Influenza A Virus, H5N2 Subtype, Influenza A Virus, H5N8 Subtype.
- enzymology : Influenza A Virus, H5N1 Subtype, Influenza A Virus, H5N2 Subtype, Influenza A Virus, H5N8 Subtype.
- epidemiology : Asia, Canada, Europe, Influenza in Birds, North America.
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus, Influenza A Virus, H5N1 Subtype, Influenza A Virus, H5N2 Subtype, Influenza A Virus, H5N8 Subtype, Neuraminidase.
- chemical , metabolism : Hemagglutinin Glycoproteins, Influenza Virus, Neuraminidase.
- virology : Animals, Wild, Influenza in Birds, Influenza, Human.
- Animals, Disease Outbreaks, Humans, Phylogeny, Poultry, Reassortant Viruses.

Abstract

UNLABELLED

During 2014, a subclade 2.3.4.4 highly pathogenic avian influenza (HPAI) A(H5N8) virus caused poultry outbreaks around the world. In late 2014/early 2015, the virus was detected in wild birds in Canada and the United States, and these viruses also gave rise to reassortant progeny, composed of viral RNA segments (vRNAs) from both Eurasian and North American lineages. In particular, viruses were found with N1, N2, and N8 neuraminidase vRNAs, and these are collectively referred to as H5Nx viruses. In the United States, more than 48 million domestic birds have been affected. Here we present a detailed structural and biochemical analysis of the surface antigens of H5N1, H5N2, and H5N8 viruses in addition to those of a recent human H5N6 virus. Our results with recombinant hemagglutinin reveal that these viruses have a strict avian receptor binding preference, while recombinantly expressed neuraminidases are sensitive to FDA-approved and investigational antivirals. Although H5Nx viruses currently pose a low risk to humans, it is important to maintain surveillance of these circulating viruses and to continually assess future changes that may increase their pandemic potential.

IMPORTANCE

The H5Nx viruses emerging in North America, Europe, and Asia pose a great public health concern. Here we report a molecular and structural study of the major surface proteins of several H5Nx influenza viruses. Our results improve the understanding of these new viruses and provide important information on their receptor preferences and susceptibilities to antivirals, which are central to pandemic risk assessment.

DOI: 10.1128/JVI.00180-16
PubMed: 27053557

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000148
to stream Main, to step Curation: 000148

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.</title>
<author><name sortKey="Yang, Hua" sort="Yang, Hua" uniqKey="Yang H" first="Hua" last="Yang">Hua Yang</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Carney, Paul J" sort="Carney, Paul J" uniqKey="Carney P" first="Paul J" last="Carney">Paul J. Carney</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mishin, Vasiliy P" sort="Mishin, Vasiliy P" uniqKey="Mishin V" first="Vasiliy P" last="Mishin">Vasiliy P. Mishin</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Guo, Zhu" sort="Guo, Zhu" uniqKey="Guo Z" first="Zhu" last="Guo">Zhu Guo</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chang, Jessie C" sort="Chang, Jessie C" uniqKey="Chang J" first="Jessie C" last="Chang">Jessie C. Chang</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E" last="Wentworth">David E. Wentworth</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gubareva, Larisa V" sort="Gubareva, Larisa V" uniqKey="Gubareva L" first="Larisa V" last="Gubareva">Larisa V. Gubareva</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name>
<affiliation><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA fwb4@cdc.gov.</nlm:affiliation>
<wicri:noCountry code="subField">USA fwb4@cdc.gov.</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:27053557</idno>
<idno type="pmid">27053557</idno>
<idno type="doi">10.1128/JVI.00180-16</idno>
<idno type="wicri:Area/Main/Corpus">000148</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000148</idno>
<idno type="wicri:Area/Main/Curation">000148</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000148</idno>
<idno type="wicri:Area/Main/Exploration">000148</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.</title>
<author><name sortKey="Yang, Hua" sort="Yang, Hua" uniqKey="Yang H" first="Hua" last="Yang">Hua Yang</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Carney, Paul J" sort="Carney, Paul J" uniqKey="Carney P" first="Paul J" last="Carney">Paul J. Carney</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mishin, Vasiliy P" sort="Mishin, Vasiliy P" uniqKey="Mishin V" first="Vasiliy P" last="Mishin">Vasiliy P. Mishin</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Guo, Zhu" sort="Guo, Zhu" uniqKey="Guo Z" first="Zhu" last="Guo">Zhu Guo</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chang, Jessie C" sort="Chang, Jessie C" uniqKey="Chang J" first="Jessie C" last="Chang">Jessie C. Chang</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E" last="Wentworth">David E. Wentworth</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gubareva, Larisa V" sort="Gubareva, Larisa V" uniqKey="Gubareva L" first="Larisa V" last="Gubareva">Larisa V. Gubareva</name>
<affiliation wicri:level="2"><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name>
<affiliation><nlm:affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA fwb4@cdc.gov.</nlm:affiliation>
<wicri:noCountry code="subField">USA fwb4@cdc.gov.</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of virology</title>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Animals, Wild (virology)</term>
<term>Asia (epidemiology)</term>
<term>Canada (epidemiology)</term>
<term>Disease Outbreaks</term>
<term>Europe (epidemiology)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (chemistry)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term>
<term>Hemagglutinin Glycoproteins, Influenza Virus (metabolism)</term>
<term>Humans</term>
<term>Influenza A Virus, H5N1 Subtype (chemistry)</term>
<term>Influenza A Virus, H5N1 Subtype (enzymology)</term>
<term>Influenza A Virus, H5N1 Subtype (genetics)</term>
<term>Influenza A Virus, H5N2 Subtype (chemistry)</term>
<term>Influenza A Virus, H5N2 Subtype (enzymology)</term>
<term>Influenza A Virus, H5N2 Subtype (genetics)</term>
<term>Influenza A Virus, H5N8 Subtype (chemistry)</term>
<term>Influenza A Virus, H5N8 Subtype (enzymology)</term>
<term>Influenza A Virus, H5N8 Subtype (genetics)</term>
<term>Influenza in Birds (epidemiology)</term>
<term>Influenza in Birds (virology)</term>
<term>Influenza, Human (virology)</term>
<term>Neuraminidase (chemistry)</term>
<term>Neuraminidase (genetics)</term>
<term>Neuraminidase (metabolism)</term>
<term>North America (epidemiology)</term>
<term>Phylogeny</term>
<term>Poultry</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Amérique du Nord (épidémiologie)</term>
<term>Animaux</term>
<term>Animaux sauvages (virologie)</term>
<term>Asie (épidémiologie)</term>
<term>Canada (épidémiologie)</term>
<term>Europe (épidémiologie)</term>
<term>Flambées de maladies</term>
<term>Glycoprotéine hémagglutinine du virus influenza ()</term>
<term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term>
<term>Glycoprotéine hémagglutinine du virus influenza (métabolisme)</term>
<term>Grippe chez les oiseaux (virologie)</term>
<term>Grippe chez les oiseaux (épidémiologie)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains</term>
<term>Phylogénie</term>
<term>Sialidase ()</term>
<term>Sialidase (génétique)</term>
<term>Sialidase (métabolisme)</term>
<term>Sous-type H5N1 du virus de la grippe A ()</term>
<term>Sous-type H5N1 du virus de la grippe A (enzymologie)</term>
<term>Sous-type H5N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H5N2 du virus de la grippe A ()</term>
<term>Sous-type H5N2 du virus de la grippe A (enzymologie)</term>
<term>Sous-type H5N2 du virus de la grippe A (génétique)</term>
<term>Sous-type H5N8 du virus de la grippe A ()</term>
<term>Sous-type H5N8 du virus de la grippe A (enzymologie)</term>
<term>Sous-type H5N8 du virus de la grippe A (génétique)</term>
<term>Virus recombinants</term>
<term>Volaille</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Neuraminidase</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
<term>Influenza A Virus, H5N2 Subtype</term>
<term>Influenza A Virus, H5N8 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term>
<term>Sous-type H5N2 du virus de la grippe A</term>
<term>Sous-type H5N8 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
<term>Influenza A Virus, H5N2 Subtype</term>
<term>Influenza A Virus, H5N8 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Asia</term>
<term>Canada</term>
<term>Europe</term>
<term>Influenza in Birds</term>
<term>North America</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Influenza A Virus, H5N1 Subtype</term>
<term>Influenza A Virus, H5N2 Subtype</term>
<term>Influenza A Virus, H5N8 Subtype</term>
<term>Neuraminidase</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Sialidase</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Sous-type H5N2 du virus de la grippe A</term>
<term>Sous-type H5N8 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term>
<term>Neuraminidase</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Sialidase</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Animaux sauvages</term>
<term>Grippe chez les oiseaux</term>
<term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Animals, Wild</term>
<term>Influenza in Birds</term>
<term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Amérique du Nord</term>
<term>Asie</term>
<term>Canada</term>
<term>Europe</term>
<term>Grippe chez les oiseaux</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Disease Outbreaks</term>
<term>Humans</term>
<term>Phylogeny</term>
<term>Poultry</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Flambées de maladies</term>
<term>Glycoprotéine hémagglutinine du virus influenza</term>
<term>Humains</term>
<term>Phylogénie</term>
<term>Sialidase</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Sous-type H5N2 du virus de la grippe A</term>
<term>Sous-type H5N8 du virus de la grippe A</term>
<term>Virus recombinants</term>
<term>Volaille</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>UNLABELLED</b>
</p>
<p>During 2014, a subclade 2.3.4.4 highly pathogenic avian influenza (HPAI) A(H5N8) virus caused poultry outbreaks around the world. In late 2014/early 2015, the virus was detected in wild birds in Canada and the United States, and these viruses also gave rise to reassortant progeny, composed of viral RNA segments (vRNAs) from both Eurasian and North American lineages. In particular, viruses were found with N1, N2, and N8 neuraminidase vRNAs, and these are collectively referred to as H5Nx viruses. In the United States, more than 48 million domestic birds have been affected. Here we present a detailed structural and biochemical analysis of the surface antigens of H5N1, H5N2, and H5N8 viruses in addition to those of a recent human H5N6 virus. Our results with recombinant hemagglutinin reveal that these viruses have a strict avian receptor binding preference, while recombinantly expressed neuraminidases are sensitive to FDA-approved and investigational antivirals. Although H5Nx viruses currently pose a low risk to humans, it is important to maintain surveillance of these circulating viruses and to continually assess future changes that may increase their pandemic potential.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>IMPORTANCE</b>
</p>
<p>The H5Nx viruses emerging in North America, Europe, and Asia pose a great public health concern. Here we report a molecular and structural study of the major surface proteins of several H5Nx influenza viruses. Our results improve the understanding of these new viruses and provide important information on their receptor preferences and susceptibilities to antivirals, which are central to pandemic risk assessment.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27053557</PMID>
<DateCompleted><Year>2017</Year>
<Month>05</Month>
<Day>03</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>06</Day>
</DateRevised>
<Article PubModel="Electronic-Print"><Journal><ISSN IssnType="Electronic">1098-5514</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>90</Volume>
<Issue>12</Issue>
<PubDate><Year>2016</Year>
<Month>06</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>Journal of virology</Title>
<ISOAbbreviation>J. Virol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.</ArticleTitle>
<Pagination><MedlinePgn>5770-5784</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1128/JVI.00180-16</ELocationID>
<Abstract><AbstractText Label="UNLABELLED">During 2014, a subclade 2.3.4.4 highly pathogenic avian influenza (HPAI) A(H5N8) virus caused poultry outbreaks around the world. In late 2014/early 2015, the virus was detected in wild birds in Canada and the United States, and these viruses also gave rise to reassortant progeny, composed of viral RNA segments (vRNAs) from both Eurasian and North American lineages. In particular, viruses were found with N1, N2, and N8 neuraminidase vRNAs, and these are collectively referred to as H5Nx viruses. In the United States, more than 48 million domestic birds have been affected. Here we present a detailed structural and biochemical analysis of the surface antigens of H5N1, H5N2, and H5N8 viruses in addition to those of a recent human H5N6 virus. Our results with recombinant hemagglutinin reveal that these viruses have a strict avian receptor binding preference, while recombinantly expressed neuraminidases are sensitive to FDA-approved and investigational antivirals. Although H5Nx viruses currently pose a low risk to humans, it is important to maintain surveillance of these circulating viruses and to continually assess future changes that may increase their pandemic potential.</AbstractText>
<AbstractText Label="IMPORTANCE">The H5Nx viruses emerging in North America, Europe, and Asia pose a great public health concern. Here we report a molecular and structural study of the major surface proteins of several H5Nx influenza viruses. Our results improve the understanding of these new viruses and provide important information on their receptor preferences and susceptibilities to antivirals, which are central to pandemic risk assessment.</AbstractText>
<CopyrightInformation>Copyright © 2016, American Society for Microbiology. All Rights Reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Hua</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Carney</LastName>
<ForeName>Paul J</ForeName>
<Initials>PJ</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mishin</LastName>
<ForeName>Vasiliy P</ForeName>
<Initials>VP</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Guo</LastName>
<ForeName>Zhu</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chang</LastName>
<ForeName>Jessie C</ForeName>
<Initials>JC</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wentworth</LastName>
<ForeName>David E</ForeName>
<Initials>DE</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gubareva</LastName>
<ForeName>Larisa V</ForeName>
<Initials>LV</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Stevens</LastName>
<ForeName>James</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA fwb4@cdc.gov.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>U54 GM062116</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>05</Month>
<Day>27</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>J Virol</MedlineTA>
<NlmUniqueID>0113724</NlmUniqueID>
<ISSNLinking>0022-538X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C109963">hemagglutinin, avian influenza A virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber>
<NameOfSubstance UI="D009439">Neuraminidase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000835" MajorTopicYN="N">Animals, Wild</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001208" MajorTopicYN="N">Asia</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002170" MajorTopicYN="N">Canada</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004196" MajorTopicYN="N">Disease Outbreaks</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005060" MajorTopicYN="N">Europe</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053124" MajorTopicYN="N">Influenza A Virus, H5N1 Subtype</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053125" MajorTopicYN="N">Influenza A Virus, H5N2 Subtype</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000071225" MajorTopicYN="N">Influenza A Virus, H5N8 Subtype</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005585" MajorTopicYN="N">Influenza in Birds</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009439" MajorTopicYN="N">Neuraminidase</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009656" MajorTopicYN="N">North America</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011200" MajorTopicYN="N">Poultry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016865" MajorTopicYN="N">Reassortant Viruses</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>01</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>04</Month>
<Day>03</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>4</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>4</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>5</Month>
<Day>4</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">27053557</ArticleId>
<ArticleId IdType="pii">JVI.00180-16</ArticleId>
<ArticleId IdType="doi">10.1128/JVI.00180-16</ArticleId>
<ArticleId IdType="pmc">PMC4886766</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Anal Biochem. 1979 Apr 15;94(2):287-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">464297</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1983 Jul 7-13;304(5921):76-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6191220</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):122-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11134934</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2006 Jun;12(6):881-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16707041</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20124702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Rev. 1992 Mar;56(1):152-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1579108</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2010 Jul;84(13):6570-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20427525</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Immunol. 2013 Dec;56(4):705-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23933511</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2003 May 10;309(2):209-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12758169</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2015 Mar;89(5):2801-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25540377</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2007 Aug 10;317(5839):825-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17690300</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 1998 Feb 14;351(9101):467-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9482437</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 1994 Jul 12;33(27):8172-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8031750</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):458-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15805601</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Sep 27;8(9):e75209</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24086467</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15299374</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1995 Feb;69(2):1099-106</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7815489</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 1997 Jun 23;233(1):224-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9201232</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17027-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15569942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Sep;87(18):10016-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23824808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1981 Jan 29;289(5796):366-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7464906</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2012 Jan 5;422(1):105-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22056389</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Influenza Other Respir Viruses. 2012 Jul;6(4):245-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22085243</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 1994 Nov 15;205(1):17-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7975212</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Curr. 2010 Mar 22;2:RRN1152</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20352039</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2005 Sep 29;353(13):1363-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16192481</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10682-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16030144</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2010 Sep 02;6(9):e1001081</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20824086</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1998 Oct 30;95(3):409-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9814710</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2007 Jul;35(Web Server issue):W375-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17452350</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2008 Sep 26;4(9):e1000161</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18818732</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2004 Mar 19;303(5665):1866-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14764887</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Apr 21;312(5772):404-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16543414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2011 Oct;7(10):e1002249</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22028647</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1997;276:307-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27754618</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):947-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16929094</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 1994 Feb;75 ( Pt 2):381-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8113759</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteins. 1989;6(4):341-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2482974</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2007;363:175-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17272842</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1981 Jan 29;289(5796):373-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6162101</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Biochem. 1992 Jul 1;207(1):335-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1628657</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 Aug;88(16):9197-207</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24899180</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2004 Aug 1;325(2):287-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15246268</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2004 Mar 19;303(5665):1838-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14764886</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Dec;86(23):12861-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22993148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17175-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19805083</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Wkly Epidemiol Rec. 2015 Mar 20;90(12):109-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25796641</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteins. 1987;2(2):111-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3447170</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Aug;86(16):8645-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22674977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Aug;85(16):8431-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21653672</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Nov;82(21):10493-501</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18715929</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Microbiol. 1994 Feb;2(2):39-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8162439</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2006 Sep 7;443(7107):45-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16915235</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11181-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11562490</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Influenza Other Respir Viruses. 2015 Sep;9(5):271-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25966311</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2015 May;21(5):886-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25898265</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2013;9(10):e1003657</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24130481</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect. 2016 Jan;72(1):52-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26143617</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2006 Feb 3;355(5):1143-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16343533</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2000 Sep;74(18):8502-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10954551</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1983 May 5-11;303(5912):35-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6843658</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15572765</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Nov;87(22):12433-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24027325</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2012 Jun 22;336(6088):1541-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22723414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17736-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19004788</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1992 Jan;11(1):49-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1740114</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Philos Trans R Soc Lond B Biol Sci. 2001 Dec 29;356(1416):1871-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11779386</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2006 Nov 16;444(7117):378-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17108965</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1996 Mar 15;271(11):6363-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8626433</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Aug;87(16):9077-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23760233</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18903-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23012478</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Géorgie (États-Unis)</li>
</region>
</list>
<tree><noCountry><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name>
</noCountry>
<country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Yang, Hua" sort="Yang, Hua" uniqKey="Yang H" first="Hua" last="Yang">Hua Yang</name>
</region>
<name sortKey="Carney, Paul J" sort="Carney, Paul J" uniqKey="Carney P" first="Paul J" last="Carney">Paul J. Carney</name>
<name sortKey="Chang, Jessie C" sort="Chang, Jessie C" uniqKey="Chang J" first="Jessie C" last="Chang">Jessie C. Chang</name>
<name sortKey="Gubareva, Larisa V" sort="Gubareva, Larisa V" uniqKey="Gubareva L" first="Larisa V" last="Gubareva">Larisa V. Gubareva</name>
<name sortKey="Guo, Zhu" sort="Guo, Zhu" uniqKey="Guo Z" first="Zhu" last="Guo">Zhu Guo</name>
<name sortKey="Mishin, Vasiliy P" sort="Mishin, Vasiliy P" uniqKey="Mishin V" first="Vasiliy P" last="Mishin">Vasiliy P. Mishin</name>
<name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E" last="Wentworth">David E. Wentworth</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/GrippeCanadaV3/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000139 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000139 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    GrippeCanadaV3
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:27053557
   |texte=   Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:27053557" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a GrippeCanadaV3

This area was generated with Dilib version V0.6.35.
Data generation: Tue Jul 7 13:36:58 2020. Site generation: Sat Sep 26 07:06:42 2020

	Corpus GrippeCanadaV3
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Corpus GrippeCanadaV3

Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.

Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki